A competitive enzyme immunoassay for screening and quantitative analysis of Tetrodotoxin (TTX) in fish and shellfish.
Tetrodotoxin (TTX) and its analogues belong to a group of neurotoxins that are produced by various marine bacteria. The toxin can accumulate in certain species of fish, different marine bivalves (clams, oysters and mussels) and gastropods. The ingestion of contaminated seafood can have fatal consequences. On the cellular level TTX causes blockage of voltage-gated sodium channels that leads to alteration of neuronal functions and muscle paralysis.
Death can occur due to heart or respiratory failure.
The Tetrodotoxin ELISA is a competitive enzyme immunoassay based on an antibody directed against TTX.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For fish and shellfish fast and efficient methods are included in the kit manual.|
|Incubation time||60 minutes|
|LOD (Detection limit)||Fish LOD 7 ng/g CCbeta 20 ng/g|
Shellfish LOD 9 ng/g CCbeta 20 ng/g
Tetrodotoxin 100%, okadaic acid 0%, saxitoxin 0%, domoic acid 0%,