Intended use:

Solid phase clean-up columns for the purification of cereal samples to detect trichothecenes. PuriTox Trichothecene can be used for the simultaneous detection of type A and B trichothecenes by GC and LC-MS/MS.

General information:

The columns reduce background interference therefore producing better chromatography and improving accuracy of results. The solid phase clean-up columns contain material to help remove interfering components or pigments from cereal samples.The toxins are extracted from the sample, filtered and passed through the clean-up column prior to analysis by GC or LC-MS/MS. The total clean-up time takes approximately 20 minutes to perform.The result is reduced background interference therefore improving the accuracy of results.

Benefit:

  • Simultaneous detection of type A and B trichothecenes by GC and LC-MS/MS.
  • Specifications
    Art. No. TC-T220
    Test format 25 solid phase clean-up columns (syringe format)
    Sample preparation A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.
    LOD (Detection limit) NIV 7.80 ng/ml (ppb), DON 1.95 ng/ml,
    FUS X 3.90 ng/ml, NEO ≤0.49 ng/ml,
    15-Acetyl DON 0.98 ng/ml,
    3-Acetyl DON 3.90 ng/ml,
    DAS ≤ 0.49 ng/ml, HT-2 ≤ 0.49 ng/ml, T-2 ≤ 0.49 ng/ml
    Validated matrices

    Cereals

    Detected analyte

    Trichothecene in cereals.

    Evaluation It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.
    E-mail linksales@r-biopharm.de
    Files
    InstructionsEnglish
    SDS2020-04_tc-t220_puritox-trichothecene-v2_safety-data-sheet_en_lowres.pdf (English)
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