SureFast® Clostridium botulinum Screening PLUS

Art. No.: F5110

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Product info

SureFast® Clostridium botulinum Screening PLUS

Art. No.: F5110

Intended use

The SureFast® Clostridium botulinum Screening PLUS is a real-time PCR for the direct, qualitative detection of specific Botulinum neurotoxins (BoNT) A, B, E and F DNA sequences of Clostridium botulinum, Clostridium baratii and Clostridium butyricum.
Each reaction contains an internal amplification control (IAC).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.


Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal

Art. NoF5110
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® Clostridium botulinum Screening PLUS real-time PCR has a limit of detection of ≤50 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.