Intended use:

The SureFast® Clostridium botulinum Screening PLUS is a real-time PCR for the direct, qualitative detection of specific Botulinum neurotoxins (BoNT) A, B, E and F DNA sequences of Clostridium botulinum, Clostridium baratii and Clostridium butyricum.
Each reaction contains an internal amplification control (IAC).

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.

Accessories:

Specifications
Art. No. F5110
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection limit) The SureFast® Clostridium botulinum Screening PLUS real-time PCR has a limit of detection of ≤50 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English
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