Intended use:

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food.

Two in ONE – STEC 4plex test kit with included DNA preparation

Benefits:

  • Integrated DNA preparation
  • Use the DNA directly for qPCR
  • Open system for all common qPCR thermocyclers

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.

Specifications
Art. No. F5265
Test format 100 reactions
Sample preparation 10 min lysis step
LOD (Detection limit) The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies.
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Files
InstructionsGerman/English
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