Intended use:

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food.

Two in ONE – STEC 4plex test kit with included DNA preparation

Benefits:

  • Integrated DNA preparation
  • Use the DNA directly for qPCR
  • Open system for all common qPCR thermocyclers

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.

Specifications
Article NumbersF5265
Test format 100 reactions
Sample preparation 10 min lysis step
LOD (Detection limit) The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies.
Files
InstructionsGerman/English
Links Links
Similar products
SureFast® STEC 4plex ONE
SureFast® STEC 4plex ONE
The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Esche...
TANBead Maelstrom™ 8 Autostage
TANBead Maelstrom™ 8 Autostage
The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation...
SureFast® Escherichia coli Serotype II 4plex
SureFast® Escherichia coli ...
The SureFast® Escherichia coli Serotype II 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific wzx gene ...

Product support

Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success.

Start typing and press Enter to search