SureFast® STEC 4plex ONE

Art. No.: F5265

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Product info

SureFast® STEC 4plex ONE

Art. No.: F5265

Intended use

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food.

Two in ONE – STEC 4plex test kit with included DNA preparation


  • Integrated DNA preparation
  • Use the DNA directly for qPCR
  • Open system for all common qPCR thermocyclers

General Information

The multiplex real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.


Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal

Art. NoF5265
Test format100 reactions
Sample preparation10 min lysis step
LOD (Detection Limit)The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies.