SureFast®STEC Screening PLUS

Art. No.: F5105

  1. Home
  2. /
  3. Products
  4. /
  5. SureFast®STEC Screening PLUS


Product info

SureFast®STEC Screening PLUS

Art. No.: F5105

Intended use

The SureFast® STEC Screening PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the virulence factors stx1 (subtype a-d) and stx2 (subtype a-g) of Escherichia coli (STEC).

Each reaction contains an internal amplification control (IAC).


General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.


Art. NoF5105
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® STEC Screening PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.