Intended use:

The SureFast® STEC Screening PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the virulence factors stx1 (subtype a-d) and stx2 (subtype a-g) of Escherichia coli.

Each reaction contains an internal amplification control (PLUS).

For DNA-preparation the use of SureFast® PREP Bacteria is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche LightCycler® 2.02, Applied Biosystems 7500 and Qiagen RotorGene Q.

Specifications
Art. No. F5105
Test format 100 reactions
Detection limit The SureFast® STEC Screening PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English
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