The test detects beef (Bos taurus), sheep (Ovis aries) and goat (Capra hircus) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation the use of SureFood® PREP Basic is recomended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 680 nm at the same time. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler® 480*, Qiagen Rotor-Gene Q, ABI PRISM, Bio-Rad CFX96, Agilent MxSeries etc.).
*note: A Color Compensation is necessary for users of the Roche LightCycler® 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test format||100 reactions|
|Detection limit||The SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC real-time PCR is developed for the detection of beef, sheep and goat DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of|
detection depends on sample matrix, processing grade, DNA preparation and DNA content.