SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC
Art. No.: S6121
The test detects beef (Bos taurus), sheep (Ovis aries) and goat (Capra hircus) DNA. Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
|For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
|LOD (Detection Limit)
|The SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC real-time PCR is developed for the detection of beef, sheep and goat DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Cross reactivity was observed in the detection system for sheep (Cy5 channel) with DNA extracts from springbok (Antidorcas marsupialis) with 6 %.