The test detects horse DNA (Equus caballus) and donkey DNA (Equus asinus) seperately. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm. The technical validation of instruments was performed on Roche Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96, BMS MIC, Agilent AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The SureFood® ANIMAL ID 3plex Horse/Donkey+IAAC real-time PCR is developed for the detection of horse- and donkey-DNA in muscle meat mixtures at a relative amount of ≥ 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|