SureFood® ANIMAL ID Horse IAAC
Art. No.: S6118
The test detects horse DNA (Equua caballus). Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent Mx3005P, Roche LightCycler® 2.0,Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96 and LTF MyGo Pro.
|For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
|LOD (Detection Limit)
|The SureFood® ANIMAL ID Horse IAAC real-time PCR is developed for the detection of horse DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.