The test detects horse DNA (Equua caballus). Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
The real-time PCR assay can be used with established realtime PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test format||100 reactions|
|Detection limit||The SureFood® ANIMAL ID Horse IAAC real-time PCR is developed for the detection of Horse DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|