Intended use:

The test detects horse DNA (Equua caballus). Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Agilent Mx3005P and on LTF MyGo Pro.

*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.

Accessories:

Specifications
Art. No. S6118
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
LOD (Detection limit) The SureFood® ANIMAL ID Horse IAAC real-time PCR is developed for the detection of horse DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English
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