SureFood® ANIMAL QUANT Beef
Art. No.: S1010
Product info
Intended use
The test detects the relative beef DNA amount (Bos taurus) to the total animal DNA content (amnions) in meat samples. Therefore the kit contains two PCR systems, one for detection of a beef specific gene (Beef detection gene) and one for the detection of an animal gene (Reference gene).
General Information
The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, Roche LightCycler® 2.0, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
Accessories
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.
| Specifications | |
|---|---|
| Art. No | S1010 |
| Test format | 2 x 50 reactions |
| Sample preparation | For DNA-preparation of meat samples the use of SureFood® PREP Basic (Art. No. S1052) is recommended. |
| LOD (Detection Limit) | The beef specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for beef DNA is 0.04 %. |
| Cross Reactivity. | Cross reactivity was observed with DNA extracts from Bison sp. (100 %) and Water buffalo (Bubalis arnee) with 0.6%. |
| Files | |
|---|---|
| Instructions | German/English |
| MSDS | Statement-MSDS-12-22-NG.pdf (English) |
