The test detects water buffalo (Bubalis arnee) and beef (Bos taurus) DNA. Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II/Roche cobas z 480 Analyzer2, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Agilent Mx3005P and Agilent AriaDx.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The SureFood® ANIMAL ID 3plex Water Buffalo/Beef+IAAC real-time PCR is developed for the detection of water buffalo and beef DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|