The test detects the relative chicken DNA amount to the total animal DNA content in meat samples. Therefore the kit contains two PCR systems, one for detection of a chicken specific gene (Gallus detection gene) and one for the detection of an animal gene (Ref reference gene).
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
|Test format||2 x 50 reactions. Thereof 50 reactions are used for the detection of the reference gene.|
|Sample preparation||For DNA preparation the kit SureFood® Basic is recomended.|
|Detection limit||The chicken specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for chicken DNA is 0.1 %.