The SureFood® ANIMAL QUANT Chicken real-time PCR detects the relative chicken (Gallus gallus) DNA amount to the total animal (amnions) DNA content in meat samples. Therefore the kit contains two PCR systems, one for detection of a chicken specific gene (Chicken detection gene) and one for the detection of an animal gene (Reference gene).
The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
|Test format||2 x 50 reactions. Thereof 50 reactions are used for the detection of the reference gene.|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||The chicken specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for chicken DNA is 0.1 %.