The test detects the relative pork DNA amount (Sus scrofa) to the total animal DNA content (amnions) in meat samples. Therefore the kit contains two PCR systems, one for detection of a pork specific gene (Sus detection gene) and one for the detection of an animal gene (reference gene).
The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Roche LightCycler® 480 II.
|Test format||2 x 50 reactions|
|Sample preparation||For DNA-preparation of meat samples the use of SureFood® PREP Basic is recommended.|
|LOD (Detection limit)||The pork specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. Forexample, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for pork DNA is 0.04 %.