Intended use:
The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference system). The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.). For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
| Article Numbers | S2061 |
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| Test format | 2 x 50 reactions |
| LOD (Detection limit) | The GT73 Canola PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Brassicacea specific reference gene are present, the relative quantitation limit for GT73 Canola DNA is 0.1 %. |
| Cross reactivity | The reference PCR system is specific for the Brassicacea family. That means that quantification is only |
| Instructions | German/English |
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