The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference system). The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.). For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
|Test format||2 x 50 reactions|
|LOD (Detection limit)||The GT73 Canola PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on|
sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For
example, if 50,000 target-sequence copies of Brassicacea specific reference gene are present, the relative
quantitation limit for GT73 Canola DNA is 0.1 %.
The reference PCR system is specific for the Brassicacea family. That means that quantification is only