Intended use:

The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference system). The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.). For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.

Specifications
Art. No. S2061
Test format 2 x 50 reactions
LOD (Detection limit) The GT73 Canola PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on
sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For
example, if 50,000 target-sequence copies of Brassicacea specific reference gene are present, the relative
quantitation limit for GT73 Canola DNA is 0.1 %.
Cross reactivity

The reference PCR system is specific for the Brassicacea family. That means that quantification is only
possible for samples in which canola material is known to be the only Brassicacea type. Therefore the GT73 Canola quantification in a mustard sample is not possible.

Files
InstructionsGerman/English
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