SureFood® GMO QUANT RoundUp Ready Soya
Art. No.: S2014
SureFood® GMO QUANT Roundup Ready Soya (RR soya) is a real-time PCR for the detection of the relative RR soya DNA amount to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR soya specific gene (Roundup Ready™ soya, GTS 40-3-2, OECD unique identifier MON-Ø4Ø32-6) and one for the detection of a soya gene (reference gene).
The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96, Bio-Rad CFX96, Bio-Rad CFX Opus, R-Biopharm RIDA®CYCLER and Agilent AriaDx.
|Test format||2x50 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.|
|LOD (Detection Limit)||The Roundup Ready Soya PCR has a limit of detection of ≤ 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed soybean.|
The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
|LOQ (Limit of quantification|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for RR soya DNA is 0.1 %.