SureFood® GMO QUANT RoundUp Ready Soya

Art. No.: S2014

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Product info

SureFood® GMO QUANT RoundUp Ready Soya

Art. No.: S2014

Intended use

The test detects the relative quantitative Roundup Ready Soya DNA amount (RR-Soya).

Therefore the kit contains two PCR systems, one specific for the Roundup Ready Soya (OECD unique identifier MON-Ø4Ø32-6) and the other one specific for Soya (reference system). The construct specific GMO detection is according to the official collection of detection methods of §64 German food law.

 

General Information

The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX96, Roche LightCycler®480 II/ Roche cobas z Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500.

Specifications
Art. NoS2014
Test format2x50 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (S1053) is recommended.
LOD (Detection Limit)The Roundup Ready Soya PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Soya specific reference gene are present, the relative quantitation limit for Roundup Ready Soya DNA is 0.1 %.
Files
InstructionsGerman/English