SureFood® GMO QUANT RR2Y Soya
Art. No.: S2029
SureFood® GMO QUANT RR2Y Soya is a real-time PCR for the detection of the relative Roundup Ready 2 Yield® Soya DNA amount (RR2Y soya) to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR2Y soya-specific gene (MON89788 soya, OECD unique identifier MON-89788-1) and one for the detection of a soya gene (reference gene).
The event specific RR2Yield-Soya detection is according to the validated method of the European Commission.
The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, Bio R-Biopharm RIDA®CYCLER und Agilent AriaDx.
|Test format||2x50 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.|
|LOD (Detection Limit)||The RR2Yield-Soya PCR has a limit of detection of ≤ 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed soybeans.|
The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
|LOQ (Limit of quantification|
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Soya specific reference gene are present, the relative quantitation limit for RR2Yield-Soya DNA is 0.1 %.