VitaFast® Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample material. Furthermore the total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) can be detected (see application note).
Ascorbic acid is a clear, odourless, cristalline solid substance which has a sour flavour. There are four stereoisomeric variants but only L-(+)-ascorbic acid (also referred to as vitamin C) is biologically active. Its vitamin activity is the most relevant property of L-ascorbic acid: humans cannot produce vitamin C and require it as part of their nutrition. A deficiency can result in the manifestation of scurvy. L-ascorbic acid and its salt, the anion L-ascorbate, will hereinafter be referred to as ascorbic acid and ascorbate, respectively.
- The method is robust and suitable for the routine determination of the ascorbic acid content in a multitude of foodstuffs and beverages.
- This test has very low detection and quantification limit.
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||Homogenisation, extraction, filtration, dilution,|
|Incubation time||Step 1 = 20 min in the dark at 20-25 °C,|
Step 2 = 30 min in the dark at 20-25 °C.
|LOD (Detection limit)||7.8 mg / 100 g (ml)|
|LOQ (Limit of quantification)|
7.8 mg / 100 g (ml)
Fruit gums, multivitamin effervescent tablets, Iron and vitamin C tablets.
|Available application notes|
Total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid)
|Detected analyte||Ascorbic acid concentration|
|Evaluation||Microtiter plate photometer 550-570 nm|