VitaFast® Vitamin C (L-Ascorbic Acid)

Art. No.: P1010

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Product info

VitaFast® Vitamin C (L-Ascorbic Acid)

Art. No.: P1010

Intended use

VitaFast® Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample material. Furthermore the total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) can be detected (see application note).

General Information

Ascorbic acid is a clear, odourless, cristalline solid substance which has a sour flavour. There are four stereoisomeric variants but only L-(+)-ascorbic acid (also referred to as vitamin C) is biologically active. Its vitamin activity is the most relevant property of L-ascorbic acid: humans cannot produce vitamin C and require it as part of their nutrition. A deficiency can result in the manifestation of scurvy. L-ascorbic acid and its salt, the anion L-ascorbate, will hereinafter be referred to as ascorbic acid and ascorbate, respectively.


  • The method is robust and suitable for the routine determination of the ascorbic acid content in a multitude of foodstuffs and beverages.
  • This test has very low detection and quantification limit.

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal

Art. NoP1010
Test formatMicrotiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparationHomogenisation, extraction, filtration, dilution,
Incubation timeStep 1 = 20 min in the dark at 20-25 °C,
Step 2 = 30 min in the dark at 20-25 °C.
LOD (Detection Limit)7.8 mg / 100 g (ml)
LOQ (Limit of quantification

7.8 mg / 100 g (ml)

Validated matrices

Fruit gums, multivitamin effervescent tablets, Iron and vitamin C tablets.

Detected analyte

Ascorbic acid concentration

Available application notes

Total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid)

EvaluationMicrotiter plate photometer 550-570 nm