Easy to conduct test in microtiter plate format for the quantitative determination of Vitamin C in food, pharmaceutical products and other sample material. Additionally L-dehydroascorbic acid can be detected (see application note).
This is an enzymatic test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in food, pharmaceutical products and other sample materials. Additionally L-dehydroascorbic acid can be detected (see application note). The kit contains all required reagents including the L-ascorbic acid standard for the standard curve. 50 reactions (plus four calibration curves) can be carried out. For evaluation a microtiter plate photometer (550 – 570 nm) is necessary. The limit of detection and the limit of quantification of the VitaFast® Vitamin C test is 7.8 mg/100g (ml). The recovery rate ranges between 95 – 105%.
|Test format||Microtiter plate with 96 wells|
|Sample preparation||Homogenisation, extraction, filtration, dilution|
|Incubation time||first: 20 min at 20 - 25 degree|
second: 30 min at 20 - 25 degree
|Detection limit||7.8 mg/100 g ( = quantification limit)|