SureFood® GMO ID 4plex Canola I

Art. No.: S2166

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Product info

SureFood® GMO ID 4plex Canola I

Art. No.: S2166

Intended use

The multiplex test detects the following DNA sequences of genetically modified canola:

  • FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8)
  • ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7)
  • Cy5 channel: T45 canola (OECD unique identifier ACS-BNØØ8-2)

The event specific detection is according to the official method of the European Commission. Each reaction contains an internal amplification control.

 

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER und Agilent Mx3005P.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS2166
Test format100 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The SureFood® GMO ID 4plex Canola I assay has a limit of detection of ca. 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.