The multiplex test detects the following DNA-sequences of genetically modified canola:
– FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9)
– VIC channel: Canola
– ROX channel: DP73496 canola (OECD unique identifier DP-Ø73496-4)
– Cy5 channel: RF3 canola (OECD unique identifier ACS-BNØØ3-6)
The detections are according to the validated method of the European Commission.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II and R-Biopharm RIDA®CYCLER.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended. SureFood® PREP Add On is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.|
|LOD (Detection limit)||The SureFood® GMO ID 4plex Canola II real-time PCR has a limit of detection of < 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.|