The multiplex test detects the following DNA sequences of genetically modified canola:
- FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8)
- ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7)
- Cy5 channel: T45 canola (OECD unique identifier ACS-BNØØ8-2)
The event specific detection is according to the official method of the European Commission. Each reaction contains an internal amplification control.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm und 660 nm (FAM, VIC, ROX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler®480 II*, Roche cobas z 480 Analyzer*, Applied Biosystems 7500, Bio-Rad CFX96 Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCycler® 480 II and the Roche cobas z 480 Analyzer. For the Color Compensation of such devices the SureCC Color Compensation Kit I (Art. No. F4009) must to be used.
|Test format||100 reactions|
|LOD (Detection limit)||The SureFood® GMO ID 4plex Canola I assay has a limit of detection of ca. 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|