SureFood® GMO ID 4plex Canola I
Art. No.: S2166
The multiplex test detects the following DNA sequences of genetically modified canola:
- FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8)
- ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7)
- Cy5 channel: T45 canola (OECD unique identifier ACS-BNØØ8-2)
The event specific detection is according to the official method of the European Commission. Each reaction contains an internal amplification control.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER und Agilent Mx3005P.
|For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
|LOD (Detection Limit)
|The SureFood® GMO ID 4plex Canola I assay has a limit of detection of ca. 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.