SureFast® Listeria monocytogenes PLUS
Art. No. F5113

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Product info

SureFast® Listeria monocytogenes PLUS

Art. No. F5113

Intended use

SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (IAC).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. TThe technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, LTF MyGo Pro, Cepheid SmartCycler® and Agilent Mx3005P.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoF5113
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® Listeria monocytogenes PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.