SureFast® Listeria monocytogenes PLUS

Art. No.: F5113

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Product info

SureFast® Listeria monocytogenes PLUS

Art. No.: F5113

Intended use

SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (IAC).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.

Specifications
Art. NoF5113
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® Listeria monocytogenes PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English