Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently used in animal production due to their excellent antibacterial and pharmacokinetic properties. They have also been used as growth promoters during the production of shrimp, poultry and pigs. Long term animal experiments have shown that the parent compounds and their metabolites have carcinogenic and mutagenic characteristics. This led to the prohibition of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also prohibited. A minimum required performance limit (MRPL) of 1000 ng/kg (ppt) for test systems was applied.
The analysis of nitrofurans is based on the detection of the tissue bound metabolites. The parent compounds are difficult to detect accurately, since they are metabolized very rapidly after treatment. The tissue bound nitrofuran metabolites however are present for a long time after administration and therefore are used for the detection of nitrofuran abuse.
R-Biopharm now offers test kits for the analysis of all Nitrofuran metabolites:
RIDASCREEN® Nitrofuran AMOZ (Art. No 3711):
Enzyme immunoassay for the analysis of AMOZ in shrimp, meat (chicken, pork, beef), liver, fish and whole egg.
RIDASCREEN® Nitrofuran AOZ (Art. No 3703):
Enzyme immunoassay for the analysis of AOZ in shrimp, meat (chicken, pork, beef),liver, fish, whole egg and milk.
RIDASREEN® Nitrofuran SEM (Art. No R3715):
Enzyme immunoassay for the analysis of SEM in shrimp, meat (chicken, pork, beef) and fish.
A new sample preparation method for shrimp allows to analyze the same sample extract in all 4 RIDASCREEN Nitrofuran ELISA tests.
Download Application Note with Validation Report