RIDASCREEN®FAST Gliadin sensitive
Art. No. R7051
Product info
Intended use
RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).
General Information
The use of wheat flour and gluten in foodstuffs is extremely common because of their heat stability and useful effects on e.g. texture, moisture retention and flavour. Gluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Coeliac disease is a permanent intolerance to gluten that results in damage to the small intestine and is reversible when gluten is avoided by diet. According to the Codex Alimentarius (CODEX STAN 118/1979) two categories for labeling of food according to the gluten content now exist:
1.) “Glutenfree” = Food products, containing < 20 mg / kg Gluten
2.) “Very low gluten” = Food products containing 20 – 100 mg / kg Gluten
The threshold of 20 mg / kg has been adopted by many national legislations in many countries.
The sample preparation using the Cocktail (patented) (R7006 / R7016) is the official R5-Mendez method according to the Codex Alimentarius and the AOAC. The faster sample preparation using the environmental-friendly Cocktail ECO (R7080) is convenient for the screening of samples. The Cocktail ECO has an extraction efficiency of approx. 70 – 110 % compared to Cocktail (patented).
Benefits:
- Based on the R5 antibody plus further antibodies against other gluten proteins.
- Different sample extractions validated depending on the Matrices (Cocktail patented (R7016), Cocktail ECO (R7080)) adapted to the specific requirements of the different samples.
- Fast and environmental-friendly Cocktail ECO (R7080) reducing sample preparation from 120 min (Cocktail patented (R7016)) to 35 min.
Patent Marking:
If the R-Biopharm product “Cocktail ECO” is used for the processing of food samples, a sulphite-containing extraction agent is used. Food inspection methods using a sulfite-containing extractant as in this product and/or corresponding detection kits are subject to the following patents of MORINAGA & Co., Ltd.: European Patent EP 2 224 239 B1, Australian Patent AU 2008 330 507 B2, United States Patent US 8 859 212 B2, Japanese Patent JP 5 451 854 B2. The patent holder has granted R-Biopharm AG a license to use, and sell products that employ, said protected technology in the above-mentioned territories.
Accessories
Dear customers,
we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.
| Specifications | |
|---|---|
| Art. No | R7051 |
| Test format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Sample preparation | Homogenization and extraction |
| Incubation time | 30 min Room temperature |
| LOD (Detection Limit) | 0.2 mg / kg (ppm) Gliadin (mean) or to 0.4 mg / kg (ppm) Gluten 0.19 – 0.21 mg / kg (ppm) Gliadin* *depending on matrix |
| LOQ (Limit of quantification | 1.25 mg / kg (ppm) Gliadin or 2.5 mg / kg (ppm) Gluten |
| Validated matrices | Gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour). |
| Detected analyte |
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| Available application notes |
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| Evaluation | Microtiter plate spectrophotometer (450 nm) |
