RIDASCREEN® Gliadin competitive (Art. No. R7021) is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough, soy sauce) which are declared as “gluten-free”. The R5 monoclonal antibody recognizes potentially toxic peptide sequences of gliadins from wheat and related prolamins from rye and barley.
The competitive enzyme immunoassay quantitates peptide fragments of prolamins from wheat (gliadins), rye (secalin) and barley (hordein). The used R5 monoclonal antibody recognizes among others the potentially toxic sequence QQPFP, which occurs repeatedly in the prolamin molecules.
The ELISA RIDASCREEN® Gliadin competitive was evaluated by 18 labs in an international collaborative study (AACCI and WGPAT) for beer, starch syrup and sourdough. The competitive ELISA is an AACC international approved method (38-55.01) and an AOAC approved Official Method of Analysis (First Action OMA 2015.05). In a second international collaborative study (ASBC) RIDASCREEN® Gliadin competitive was evaluated by 15 labs for 5 different beers. The competitive ELISA is an ASBC international approved method (Beer-49).
In addition, the RIDASCREEN® Gliadin competitive is approved by AOECS (Standard R5 ELISA for hydrolyzed food), MEBAK (Method 2.6.5 beer analysis) and TTB (ruling number 2012-2 for beer).
- AOAC Official First Action Method - OMA 2015.05
- AACCI - Approved Method 38-55.01
- ASBC Methods of Analysis - Beer-49
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|