SureFood® ALLERGEN Walnut

Art. No.: S3607

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Product info

SureFood® ALLERGEN Walnut

Art. No.: S3607

Intended use

The SureFood® ALLERGEN Walnut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific walnut (Juglans regia and Juglans nigra) DNA sequences according to directive (EC) 1169/2011.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg walnut / kg food sample and a detection system including a standard series are required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS3607
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Walnut PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1