Intended use:

The test detects Juglans regia (walnut) DNA according to directive (EC) 1169/2011 and Juglans nigra (eastern black walnut) DNA qualitatively and/or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg walnut / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.

General information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

Specifications
Art. No. S3607
Test format 100 reactions
LOD (Detection limit) The SureFood® ALLERGEN Walnut PCR has a limit of detection of ≤ 0.4 mg / kg.
Files
InstructionsGerman/English
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