Intended use:

The SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific Peanut (Arachis hypogaea), Hazelnut (Corylus maxima) and Walnut (Juglans regia, Juglans nigra) DNA sequences according to directive (EC) 1169/2011.

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.

Accessories:

Specifications
Article NumbersS3402
Test format 100 reactions
Sample preparation For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
LOD (Detection limit) ≤ 1 mg / kg peanut
≤ 0.4 mg / kg hazelnut
≤ 0.4 mg / kg walnut

Evaluation Real-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm)
Files
InstructionsGerman/English
MSDS (English)
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