The multiplex real-time PCR test detects DNA of peanut, hazelnut and walnut according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification control (IAC).
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. Internal validation was performed on Roche LightCycler® 480 II2, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
|Test format||100 reactions|
|LOD (Detection limit)||≤ 1 mg / kg peanut|
≤ 0.4 mg / kg hazelnut
≤ 0.4 mg / kg walnut
|Evaluation||Real-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm)|