The multiplex test detects DNA of peanut, hazelnut and walnut according to directive (EC) 1169/2011. Each reaction contains an internal amplification control.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. Internal validation was performed on Roche LightCycler® 480 II2, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
|Test format||100 reactions|
|LOD (Detection limit)||The SureFood®ALLERGEN Peanut/Hazelnut/Walnut+IAC PCR using SureFood® PREP Advanced, protocol 1 has a limit of detection of:|
≤ 1 mg / kg Peanut
≤ 0.4 mg / kg Hazelnut
≤ 0.4 mg / kg Walnut
The limit of detection of the complete quantitative determination (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.