Art. No.: RBRP183 / RBRP183B

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Product info


Art. No.: RBRP183 / RBRP183B

Intended use

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.


  • Single extraction method.
  • Helps remove matrix interference.
  • Uses monoclonal antibody.
  • Extraction uses buffer and enzymes.
  • Can be used with pigmented samples.
  • Can be stored at 21 – 25 °C.
  • The columns are suitable for testing a wide range of commodities.



Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st June 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com/rbr.

Art. NoRBRP183 / RBRP183B
Test formatRBRP183 = 10 immunoaffinity columns with 10 ml format.
RBRP183B = 50 immunoaffinity columns with 10 ml format.
Capacity = 0.45 µg biotin, 0.5 µg folic acid, 1 µg vitamin B12.
Sample preparationSample is extracted using buffer and enzymes, filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.

Biotin, 25 ng / ml
Folic acid, 10 ng / ml
Vitamin B12, 5 ng / ml
Detected analyte

Biotin, folic acid and vitamin B12

Available application notes

Methods are available for a range of commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS system