Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
- Single extraction method.
- Helps remove matrix interference.
- Uses monoclonal antibody.
- Extraction uses buffer and enzymes.
- Can be used with pigmented samples.
- Can be stored at 21 – 25 °C.
- The columns are suitable for testing a wide range of commodities.
|Art. No.||RBRP183 / RBRP183B|
|Test format||RBRP183 = 10 immunoaffinity columns with 10 ml format.|
RBRP183B = 50 immunoaffinity columns with 10 ml format.
Capacity = 0.45 µg biotin, 0.5 µg folic acid, 1 µg vitamin B12.
|Sample preparation||Phosphate buffer, pepsin, α-amylase, potassium cyanide solution andsodium ascorbate solution|
|Incubation time||90 minutes prior to detection by HPLC or LC-MS/MS|
|LOD (Detection limit)||Biotin: 25 ng / ml|
Folic acid: 10 ng / ml
Vitamin B12: 5 ng / ml
Biotin, cyanocobalamin, folic acid
|Evaluation||HPLC or LC-MS/MS system|