The SureFast® Escherichia coli Serotype I 4plex is a multiplex real-time PCR kit for the direct, qualitative detection and differentiation of specfifc wzx gene DNA sequences of Escherichia coli O121, O26 and O103.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Applied Biosystems 7500 and R-Biopharm RIDA®CYCLER.
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA).
For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal
congen.de/information.
Specifications
Art. No
F5167
Test format
100 reactions
Sample preparation
For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.
LOD (Detection Limit)
The SureFast® Escherichia coli Serotype I 4plex real-time PCR has a limit of detection of < 5 DNA copies.