The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium.
Each reaction contains an internal amplification control (IAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.|
To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
|LOD (Detection limit)||The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex (R&D Version) real-time PCR has a limit of detection of <= 5 DNA copies.|
Cross reactivity was observed with DNA extracts from Salmonella Amsterdam, Salmonella Blegdam, Salmonella Kiel, Salmonella Moscow and Salmonella Nitra in the detection system for Salmonella Enteritidis.