Intended use:

The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex (R&D Version) is a real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium.

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.

Accessories:

Specifications
Art. No. F5166
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.
To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
Detection limit The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex (R&D Version) real-time PCR has a limit of detection of <= 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Cross reactivity

Cross reactivity was observed with DNA extracts from Salmonella Amsterdam, Salmonella Blegdam, Salmonella Moscow and Salmonella Nitra in the detection system for Salmonella Enteritidis.

Files
InstructionsGerman/English
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