The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex (R&D Version) is a real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.|
To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
|Detection limit||The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex (R&D Version) real-time PCR has a limit of detection of <= 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|
Cross reactivity was observed with DNA extracts from Salmonella Amsterdam, Salmonella Blegdam, Salmonella Moscow and Salmonella Nitra in the detection system for Salmonella Enteritidis.