The real-time PCR test detects DNA ofnmollusc according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification control (IAC).
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|LOD (Detection limit)||≤ 0.4 mg / kg|
|Evaluation||Real-time PCR instrument with two detection channels (510 nm and 580 nm)|