The test detects mollusc DNA according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification control.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.|
|Detection limit||The SureFood® ALLERGEN Mollusc PCR has a limit of detection of ≤ 0.4 mg / kg. The limit of detection of the complete assay (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.|