Intended use:

The real-time PCR test detects DNA of fish according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

General information:

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg fish / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.

The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.

Accessories:

Specifications
Art. No. S3610
Test format 100 reactions
LOD (Detection limit) ≤ 1 mg / kg
LOQ (Limit of quantification)

4 mg / kg using SureFood® PREP Advanced, protocol 1

Cross reactivity

Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).

Evaluation Real-time PCR instrument with two detection channels (510 nm and 580 nm)
E-mail linksales@r-biopharm.de
Files
InstructionsGerman/English
SDSerklaerung-zu-congen-msds_2018-12.pdf (German)
confirmation-congen-msds_2018-12.pdf (English)
Similar products
RIDASCREEN®FAST Crustacean
RIDASCREEN®FAST Crustacean
RIDASCREEN®FAST Crustacean is a sandwich enzyme immunoassay for the quantitative analysis of crustaceans which can be present as ingredient or cont...
Lateral Flow Crustacean
Lateral Flow Crustacean
The Lateral Flow Crustacean assay is used for the qualitative detection of crustacean residues in environmental / surface samples (e.g. in food pro...
SureFood® ALLERGEN Molluscs
SureFood® ALLERGEN Molluscs
The real-time PCR test detects DNA ofnmollusc according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification...

Start typing and press Enter to search