The test detects fish DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg fish / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Detection limit||The SureFood® ALLERGEN Fish PCR has a limit of detection of ≤ 1 mg / kg. The limit of quantification is 4 mg / kg using SureFood® PREP Advanced, protocol 1. The limit of detection and limit of quantification were determined using the SureFood® QUANTARD Allergen 40 (matrix: corn flour).|
Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).