SureFood® ALLERGEN Fish

Art. No.: S3610

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SureFood® ALLERGEN Fish

Art. No.: S3610

Intended use

The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg fish / kg food sample and a detection system including a standard series is required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDACYCLER and Agilent AriaDx.


Art. NoS3610
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Fish PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

4 mg / kg using SureFood® PREP Advanced, protocol 1

Cross Reactivity.

Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).