Intended use:

The test detects fish DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg fish / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.

Accessories:

Specifications
Art. No. S3610
Test format 100 reactions
Detection limit The SureFood® ALLERGEN Fish PCR has a limit of detection of ≤ 1 mg / kg. The limit of quantification is 4 mg / kg using SureFood® PREP Advanced, protocol 1. The limit of detection and limit of quantification were determined using the SureFood® QUANTARD Allergen 40 (matrix: corn flour).
Cross reactivity

Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).

Files
InstructionsGerman/English
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