SureFood® ALLERGEN Crustaceans

Art. No.: S3612

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SureFood® ALLERGEN Crustaceans

Art. No.: S3612

Intended use

The SureFood® ALLERGEN Crustaceans is a real-time PCR for the direct, qualitative and / or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to directive (EC) 1169/2011. The crustacean family contains about 44,000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg crustaceans / kg food sample and a detection system including a standard series are required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, Agilent AriaDx and Agilent Mx3005P.


Art. NoS3612
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Crustaceans PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1

Cross Reactivity.

Cross reactivity was observed with DNA extracts from abalone (Haliotis spp.) with 100 %.