This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens NOS terminator DNA sequence and for detection of the 34S FMV promoter DNA sequence are applied. Additionally the kit contains an internal inhibition control.
The kit is according to German Food Law § 64 for the detection of genetically modified DNA sequences.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time (Rotor-Gene Q, Stratagene MxSeries, BioRad CFX96, Roche LightCycler® 480* etc.).
The LightCycler® 480 needs to be adjusted with the SureCC Color Compensation Kit I (F4009).
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.|
|LOD (Detection limit)||≤ 5 DNA copies|