Intended use:

The SureFood® GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences:

  •  FAM channel: MON810 corn (OECD unique identifier MON-ØØ81Ø-6)
  • VIC/HEX channel: TC1507 corn (OECD unique identifier DAS-Ø15Ø7-1)
  • ROX channel: NK603 corn (OECD unique identifier MON-ØØ6Ø3-6)
  • Cy5 channel: MON89034 corn (OECD unique identifier MON-89Ø34-3)

General information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.

Accessories:

Specifications
Art. No. S2170
Test format 100 reactions
Sample preparation For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended. SureFood® PREP Add On is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection limit) The SureFood® GMO ID 4plex Corn I real-time PCR has a limit of detection of < 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed corn grain.
Files
InstructionsGerman/English
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