SureFood® ALLERGEN Hazelnut

Art. No.: S3602

  1. Home
  2. /
  3. Products
  4. /
  5. SureFood® ALLERGEN Hazelnut

Analytes

Product info

SureFood® ALLERGEN Hazelnut

Art. No.: S3602

Order now

Intended use

The real-time PCR test detects DNA of hazelnut (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg hazelnut / kg food sample and a detection system including a standard series is required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent AriaDx, Applied Biosystems 7500, Bio Molecular Systems MIC, Bio-Rad CFX96, LTF MyGo Pro, Qiagen Rotor-Gene Q, Roche cobas® z 480 Analyzer and Roche LightCycler® 480 II.

Accessories

Specifications
Art. NoS3602
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Hazelnut PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1

EvaluationReal-time PCR instrument with two detection channels (510 nm and 580 nm)