RIDASCREEN®FAST Gliadin
Art. No. R7002
Product info about RIDASCREEN®FAST Gliadin
Intended use
Fast ELISA test method for gluten detection!
Ensures a safe and fast quantitative analysis of prolamins from wheat (gliadin), rye (secalin) and barley (hordein) in food.
RIDASCREEN®FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) . The used specific monoclonal antibody (R5) detects the amino acid sequences in the prolamin fraction of gluten that are toxic to coeliac disease patients. However, it does not react with epitopes of prolamins from oats which are tolerable for most celiac patients. Hence, it can be used to detect wheat, rye and barley gluten in oat products for gluten-free labelling purposes.
The sample preparation using the Cocktail (patented) (Art. No. R7006 / R7016) corresponds to the official R5-Mendéz method according to the Codex Alimentarius and the AOAC. The faster sample preparation using the environmental-friendlier Cocktail ECO (Art. No. R7080) is convenient for the screening of samples. The Cocktail ECO has an extraction efficiency of approx. 70 – 110 % compared to Cocktail (patented).
Benefits:
- Fast ELISA test procedure
- Kit uses the R5 antibody recommended by the Codex Alimentarius
- Comparable to reference method (Codex Alimentarius) when using the Méndez-Cocktail commercialized as Cocktail (patented)
General Information
The use of gluten in foodstuffs is extremely common because of its useful effects on e.g. texture, moisture retention and flavor. However, gluten intolerance disorders like coeliac disease require a permanent gluten-free diet to avoid clinical symptoms. According to the Codex Alimentarius (CODEX STAN 118-1979) foods for special dietary use for persons intolerant to gluten may contain up to 20 mg/kg gluten to be tolerated by celiac patients. Foods containing < 20 mg/kg gluten can be labelled “gluten-free”.
The threshold of 20 mg/kg has been adopted by national legislations in many countries. Gluten of wheat, rye and barley is a mixture of prolamin and glutelin proteins. Prolamins from wheat are named gliadins. The prolamine content of wheat gluten is defined as 50 % (CODEX STAN 118-1979). R5-based ELISA methods, which have been calibrated against gliadin (prolamin part of wheat gluten), therefore need to be multiplied by a factor of 2 to calculate the gluten concentration.
Following extraction solutions are validated for this method:
- Cocktail (patented) (Art. No. R7006 / R7016)
- RIDA® Cocktail ECO (Art. No. R7080) *
Additional extraction solutions are:
- RIDA® Extraction Solution (colorless) (Art. No. 7098)
- 60 % ethanol
* Patent Marking:
If the R-Biopharm product “Cocktail ECO” is used for the processing of food samples, a sulphite-containing extraction agent is used. Food inspection methods using a sulfite-containing extractant as in this product and/or corresponding detection kits are subject to the following patents of MORINAGA & Co., Ltd.: European Patent EP 2 224 239 B1, Australian Patent AU 2008 330 507 B2, United States Patent US 8 859 212 B2, Japanese Patent JP 5 451 854 B2. The patent holder has granted R-Biopharm AG a license to use, and sell products that employ, said protected technology in the above-mentioned territories.
Accessories
- Cocktail (patented)
- Cocktail ECO
- RIDA® Extraction Solution (colorless)
- Set of 3 processed Gliadin Assay Controls
Dear customers,
we provide the documents for our products in an electronic format which include the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01. January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.
| Specifications | |
|---|---|
| Art. No | R7002 |
| Test format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Sample preparation | Homogenization and extraction |
| Incubation time | 30 min Room temperature |
| LOD (Detection Limit) | Cocktail (patented) 0.50 mg/kg (ppm) Gliadin (mean) or to 1.0 mg/kg (ppm) Gluten Cocktail ECO 0.52 mg/kg (ppm) Gliadin (mean) or to 1.04 mg/kg (ppm) Gluten (depending on matrix) |
| LOQ (Limit of quantification | 5 mg/kg (ppm) Gliadin or 10 mg/kg (ppm) Gluten |
| Validated matrices | Contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and spices as well as in processed food like noodles, ready-to-serve meals, bakery products, sausages, beverages and ice cream |
| Detected analyte | • The main epitope of the R5 antibody is the amino acid sequence QQPFP which is part of many celiac-toxic sequences. |
| Available application notes | • Sample preparation for processed food with the RIDA® Extraction Solution (colorless) (R7098) – only after validation. • Sample preparation for raw materials with ethanol. • Sample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
