EASI-EXTRACT® T-2 and HT-2
Art. No. RBRP43 / RBRP43B
Product info
Intended use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of T-2 and HT-2 toxins in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. T-2 and HT-2 toxins require to be derivatised when analysed by HPLC.
Benefits:
- Can be used for the analysis of certain metabolites and masked mycotoxins.
- The columns give excellent recoveries and meet EU performance criteria. In addition columns have low % RSD and high capacity.
- The columns are wide format allowing the sample to flow easily by gravity.
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Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st June 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com/rbr.
| Specifications | |
|---|---|
| Art. No | RBRP43 / RBRP43B |
| Test format | 10 columns (3 ml format) (RBRP43), 50 columns (3 ml format) (RBRP43B) |
| Sample preparation | A representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column. |
| LOD (Detection Limit) | Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. T-2 0.05 ng/ml HT-2 0.05 ng/ml |
| Detected analyte | T-2 and HT-2. May also cross react with other metabolites and masked mycotoxins. |
| Available application notes | Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information. |
| Evaluation | HPLC or LC-MS/MS. |
| Approvals | The product meets all Official Method recovery and capacity specifications. |
