Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of T-2 and HT-2 toxins in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. T-2 and HT-2 toxins require to be derivatised when analysed by HPLC.
- Can be used for the analysis of certain metabolites and masked mycotoxins.
- The columns give excellent recoveries and meet EU performance criteria. In addition columns have low % RSD and high capacity.
- The columns are wide format allowing the sample to flow easily by gravity.
|Test format||10 columns (3 ml format) (RBRP43),|
50 columns (3 ml format) (RBRP43B)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 sec|
|LOD (Detection limit)||T-2 Toxin: 0.05 ng/ml (ppt), |
HT-2 Toxin: 0.05 ng/ml
Feed and food.
T-2 und HT-2 Toxin in food and feed.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|