Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of T-2 and HT-2 toxins in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. T-2 and HT-2 toxins require to be derivatised when analysed by HPLC.
|Art. No.||P43 / P43B|
|Test format||10 wide format immunoaffinity columns (P43)|
50 wide format immunoaffinity columns (P43B)
|Detection limit||LOD: T-2: 0.05 ng/ml, HT-2: 0.05 ng/ml|