Molecularly Imprinted Polymer (MIP) columns for use in conjunction with an HPLC for detection of patulin in apple juice and apple purée.
The procedure is based on molecularly imprinted polymer (MIP) technology, which makes the test specific, sensitive, rapid and simple to perform. The columns contain a molecularly imprinted polymer specific to the toxin of interest. Following extraction of the toxin the sample extract is centrifuged, filtered, and passed through the MIP column. Any toxin which is present in the sample is retained by the MIP within the column. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC.
- Simple extraction and test procedure.
- Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography interference.
- The columns are suitable for testing a wide range of commodities.
|Art. No.||RBRP250/ RBRP250B|
|Test format||RBRP250: 10 columns (3 ml format),|
RBRP250B: 50 columns (3 ml format)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 - 50 g dependent on method used) of this is removed and extracted.|
|LOD (Detection limit)||0.06 ng/kg (ppt)|
Apple juice and apple purée.
Patulin in apple juice and apple purée.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|