The test detects almond DNA (Prunus dulcis) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg almond / kg food sample and a detection system including a standard dilution is required.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.
|Test format||100 reactions|
|Sample preparation||For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.|
|LOD (Detection limit)||The SureFood® ALLERGEN Almond PCR has a limit of detection of ≤ 0.4 mg / kg. |
The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1.