SureFood® ALLERGEN Almond

Art. No.: S3604

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Product info

SureFood® ALLERGEN Almond

Art. No.: S3604

Intended use

The real-time PCR test detects DNA of almond (Prunus dulcis) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg almond / kg food sample and a detection system including a standard series is required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.

Accessories

Specifications
Art. NoS3604
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Almond PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1

EvaluationReal-time PCR instrument with two detection channels (510 nm and 580 nm)