SureFood® ALLERGEN Sesame
Art. No.: S3608
The real-time PCR test detects DNA of sesame (Sesamum indicum) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg sesame / kg food sample and a detection system including a standard series is required.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
|Test format||100 reactions|
|Sample preparation||For DNA preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.|
|LOD (Detection Limit)||The SureFood® ALLERGEN Sesame PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.|
|LOQ (Limit of quantification|
The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1.