Intended use:

The SureFood® ALLERGEN Pine Nut is a real-time PCR for the direct, qualitative detection of specific pine nut (Pinus spp.) DNA sequences in food.
Each reaction contains an internal amplification control (IAC).

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.

Accessories:

Specifications
Article NumbersS3624
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection limit) The SureFood® ALLERGEN Pine Nut PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.
Files
Instructions
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