The SureFood® ALLERGEN Pine Nut is a real-time PCR for the direct, qualitative detection of specific pine nut (Pinus spp.) DNA sequences in food.
Each reaction contains an internal amplification control (IAC).
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.|
|LOD (Detection limit)||The SureFood® ALLERGEN Pine Nut PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.|