This multiplex-test detects DNA sequences of corn, soya, canola and cotton.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time (Rotor-Gene Q, Agilent MxSeries, BioRad CFX96, ABI 7500, Roche LightCycler® 480 etc.).
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (S1052)and for highly processed food and feed the use of SureFood® PREP Advanced (S1053) is recommended.|
|LOD (Detection limit)||The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton real-time PCR has a limit of detection of < 4 mg/kg. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|
The canola PCR system is specific for the Brassicacea family. That means samples with mustard are also detected in the canola channel.