This multiplex-test detects DNA sequences of corn, soya and canola. Additionally the kit contains an internal amplification control.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 660 nm (FAM, VIC, ROX and Cy5) at the same time (Rotor-Gene Q, Agilent MxSeries, BioRad CFX96, ABI 7500, Roche LightCycler® 480 II etc.).
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (S1053) is recommended.|
|LOD (Detection limit)||The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC real-time PCR has a limit of detection of ≤ 4 mg/kg.|
The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA
The canola PCR system is specific for the Brassicacea family. That means samples with mustard are also detected in the canola channel.