The SureFast® Salmonella PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Salmonella spp.. The SureFast® Salmonella PLUS real-time PCR kit has been validated and certified in combination with the SureFast® PREP Salmonella kit by AOAC (licence number: 041103).
Each reaction contains an internal amplification control (IAC).
The real-time PCR assay can be used with real-time PCR instruments, equipped for detection of two fluorescence emissions in channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Cepheid SmartCycler®, BMS MIC, LTF MyGo Pro and Qiagen Rotor-Gene Q.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Salmonella or SureFast® Speed PREP is recommended.|
|LOD (Detection limit)||The SureFast® PATHOGEN Salmonella PLUS real-time PCR has a limit of detection of <5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|