SureFood® ALLERGEN Buckwheat
Art. No.: S3620
Product info
Intended use
The SureFood® ALLERGEN Buckwheat is a real-time PCR for the direct, qualitative and / or quantitative detection of specific buckwheat (Fagopyrum esculentum) DNA sequences.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg buckwheat / kg food sample and a detection system including a standard series is required.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96/Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
Accessories
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.
| Specifications | |
|---|---|
| Art. No | S3620 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® ALLERGEN Buckwheat PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1. |
| Files | |
|---|---|
| Instructions | German/English |
| MSDS | Statement-MSDS-12-22-NG.pdf (English) |
