ELISA

Saggi immunologici efficienti per l’analisi degli alimenti

Tecnologie

ELISA

L’ELISA (saggio immuno-assorbente legato ad un enzima) è un metodo d’analisi basato sull’impiego di anticorpi. È una tecnologia molto diffusa, caratterizzata da alta sensibilità, affidabilità e rapidità di esecuzione.

Con i test ELISA, R-Biopharm può contare su un metodo analitico consolidato in grado di rispondere alle esigenze dei moderni laboratori. I test RIDASCREEN® mostrano un’elevata sensibilità e robustezza. Per lo screening rapido di varie materie prime offriamo i test RIDASCREEN®FAST. Questi test consentono di analizzare campioni di alimenti e di mangimi entro un massimo di 30 minuti compresa la preparazione del campione.

Principio del test ELISA

Gli anticorpi sono proteine prodotte nelle plasmacellule dei vertebrati, come parte del sistema immunitario adattativo contro le strutture (antigeni) che vengono riconosciute come estranee all’organismo. Gli anticorpi si legano ai rispettivi antigeni secondo uno schema distintivo di interazioni ioniche e idrofobiche, legami idrogeno e forze di Van der Waals. L’interazione tra anticorpo e antigene è selettiva e altamente specifica, simile a quella di una serratura con la propria chiave.

Il saggio immunoenzimatico (ELISA) si basa su questo riconoscimento selettivo e specifico anticorpo-antigene. Sono stati realizzati vari tipi di test ELISA, sia qualitativi che quantitativi. L’esecuzione di un test ELISA prevede l’uso di almeno un anticorpo specifico per ogni particolare antigene. Il principio di fondo è che una di queste componenti immunologiche venga immobilizzata su una fase solida, le cavità di una micropiastra. L’analita prelevato dal campione interagisce con il sistema anticorpo-antigene. Questa interazione può essere visualizzata mediante enzimi, legati a anticorpi o antigeni secondari, e indicano se si è creato il legame anticorpo-antigene. Si aggiunge un substrato che viene convertito dal complesso enzima-anticorpo producendo un cambiamento della colorazione, che è misurabile con uno spettrofotometro.

ELISA a sandwich ed ELISA competitivo

Mentre un ELISA a sandwich rileva solo le proteine integre e grossi frammenti con almeno due siti di legame, un ELISA competitivo è in grado di individuare frammenti proteici grossi e piccoli con solo un sito di legame. Per questo motivo l’ELISA competitivo può essere impiegato anche per prodotti alimentari altamente lavorati.

Buone prassi per il test ELISA

Il manuale di buone prassi per il test ELISA offre una panoramica completa per analisti principianti ed esperti al fine di migliorare la qualità delle analisi effettuati mediante test ELISA. È pensato per orientare gli utilizzatori alla creazione di condizioni generali e d’utilizzo soddisfacenti, indispensabili per ottenere buone prestazioni durante l’utilizzo dei kit ELISA di R-Biopharm.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
EuroProxima Plus Bovine Rennet Whey

EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5171BRW
EuroProxima Plus Cheese Fraud

EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5171BKCC
EuroProxima Plus Lactoferrin

EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5091LFER
ELISA-TEK™ Cooked Meat Species Kit

Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ …

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96 determinations 5106**
ELISA-TEK™ Raw Meat Species Kit

Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: …

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96 determinations 5105**
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Egg

RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R6411
RIDASCREEN®FAST Gliadin sensitive

RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7051
RIDASCREEN® Gliadin

RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7001
RIDASCREEN®FAST Cashew

The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6872
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® T-2 / HT-2 Toxin

RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3805
RIDASCREEN® Zearalenon

RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R1401
RIDASCREEN® DON

RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R5906
RIDASCREEN® T-2 Toxin

RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3801
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ProductDescriptionNo. of tests/amountArt. No.
EuroProxima Medroxyprogesterone Acetate

A competitive enzyme immunoassay for screening and quantitative analysis of medroxyprogesterone acetate (MPA) in bovine kidney fat.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5131MPA
EuroProxima Trenbolone

This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081TRENBO
EuroProxima Florfenicol-amine

A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091FLOA
EuroProxima Neomycin

A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5111NEO
EuroProxima Oxytetracycline

A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091OTC
EuroProxima Tylosin

EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151TYL
EuroProxima Virginiamycin

EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151VIG
EuroProxima Fluoroquinolones

EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUQG
EuroProxima Fluoroquinolones II

EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUQII
EuroProxima Promazine, generic

EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201PROM
EuroProxima Malachite Green Total/Crystal Violet

EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5161MGT
EuroProxima Azaperone-Azaperol

EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201AZA
EuroProxima Carazolol

EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5201CARA
EuroProxima Corticosteroid

This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081COR
EuroProxima Progesterone

This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081PROG
EuroProxima Stanozolol

This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081STAN
EuroProxima Triamcinolone

This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081TRIA
EuroProxima Saxitoxin

EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5191SAXI
EuroProxima Lincomycin

A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5151LIN
EuroProxima Gentamicin

A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5111GEN
EuroProxima Flumequine

EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5101FLUM
EuroProxima Erythromycin

EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151ERY
EuroProxima Domoic Acid

EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5191DOMO
EuroProxima Dimetridazole

EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5091DIME
EuroProxima Colistin

A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5151COL
EuroProxima Ivermectin

EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5141IVER
EuroProxima Diethylstilbestrol (DES)

A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5081DES
EuroProxima Penicillin

A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices.

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Microtiter plate with 96 wells (12 strips with 8 wells each) 5091PEN
RIDASCREEN® Tetracyclin

RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, …

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3505
RIDASCREEN® Chloramphenicol

RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), …

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1511
RIDASCREEN® Sulfamethazin

RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3011
RIDASCREEN® Streptomycin

RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3104
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