ELISA
Saggi immunologici efficienti per l’analisi degli alimenti
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Tecnologie
ELISA
L’ELISA (saggio immuno-assorbente legato ad un enzima) è un metodo d’analisi basato sull’impiego di anticorpi. È una tecnologia molto diffusa, caratterizzata da alta sensibilità, affidabilità e rapidità di esecuzione.
Con i test ELISA, R-Biopharm può contare su un metodo analitico consolidato in grado di rispondere alle esigenze dei moderni laboratori. I test RIDASCREEN® mostrano un’elevata sensibilità e robustezza. Per lo screening rapido di varie materie prime offriamo i test RIDASCREEN®FAST. Questi test consentono di analizzare campioni di alimenti e di mangimi entro un massimo di 30 minuti compresa la preparazione del campione.
Principio del test ELISA
Gli anticorpi sono proteine prodotte nelle plasmacellule dei vertebrati, come parte del sistema immunitario adattativo contro le strutture (antigeni) che vengono riconosciute come estranee all’organismo. Gli anticorpi si legano ai rispettivi antigeni secondo uno schema distintivo di interazioni ioniche e idrofobiche, legami idrogeno e forze di Van der Waals. L’interazione tra anticorpo e antigene è selettiva e altamente specifica, simile a quella di una serratura con la propria chiave.
Il saggio immunoenzimatico (ELISA) si basa su questo riconoscimento selettivo e specifico anticorpo-antigene. Sono stati realizzati vari tipi di test ELISA, sia qualitativi che quantitativi. L’esecuzione di un test ELISA prevede l’uso di almeno un anticorpo specifico per ogni particolare antigene. Il principio di fondo è che una di queste componenti immunologiche venga immobilizzata su una fase solida, le cavità di una micropiastra. L’analita prelevato dal campione interagisce con il sistema anticorpo-antigene. Questa interazione può essere visualizzata mediante enzimi, legati a anticorpi o antigeni secondari, e indicano se si è creato il legame anticorpo-antigene. Si aggiunge un substrato che viene convertito dal complesso enzima-anticorpo producendo un cambiamento della colorazione, che è misurabile con uno spettrofotometro.
ELISA a sandwich ed ELISA competitivo
Mentre un ELISA a sandwich rileva solo le proteine integre e grossi frammenti con almeno due siti di legame, un ELISA competitivo è in grado di individuare frammenti proteici grossi e piccoli con solo un sito di legame. Per questo motivo l’ELISA competitivo può essere impiegato anche per prodotti alimentari altamente lavorati.
Buone prassi per il test ELISA
Il manuale di buone prassi per il test ELISA offre una panoramica completa per analisti principianti ed esperti al fine di migliorare la qualità delle analisi effettuati mediante test ELISA. È pensato per orientare gli utilizzatori alla creazione di condizioni generali e d’utilizzo soddisfacenti, indispensabili per ottenere buone prestazioni durante l’utilizzo dei kit ELISA di R-Biopharm.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Plus Bovine Rennet Whey |
EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BRW |
EuroProxima Plus Cheese Fraud |
EuroProxima Plus Cheese Fraud is a competitive enzyme immunoassay for screening of the presence of bovine milk in cheese of other species and sources. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5171BKCC |
EuroProxima Plus Lactoferrin |
EuroProxima Plus Lactoferrin is a competitive enzyme immunoassay for screening and quantitative analysis of lactoferrin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091LFER |
ELISA-TEK™ Cooked Meat Species Kit |
Assay for the positive identification of species content (various) in cooked samples: ELISA-TEK™ Cooked Mixed Species Kit: customized (Art. No. 510601) ELISA-TEK™ Cooked Meat 3 Species Kit: beef, pork, poultry (Art. No. 510603) ELISA-TEK™ … Continua a leggere |
96 determinations | 5106** |
ELISA-TEK™ Raw Meat Species Kit |
Assay for the positive identification of species content (various) in raw samples: ELISA-TEK™ Raw Mixed Species Kit: customized (Art. No. 510501) ELISA-TEK™ Raw 3 Species Kit: cow, pig, poultry (Art. No. 510503) ELISA-TEK™ Raw 4 Species Kit: … Continua a leggere |
96 determinations | 5105** |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® Egg |
RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, … Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R6411 |
RIDASCREEN®FAST Gliadin sensitive |
RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour). Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7051 |
RIDASCREEN® Gliadin |
RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative … Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7001 |
RIDASCREEN®FAST Cashew |
The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice … Continua a leggere |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6872 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® T-2 / HT-2 Toxin |
RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3805 |
RIDASCREEN® Zearalenon |
RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R1401 |
RIDASCREEN® DON |
RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R5906 |
RIDASCREEN® T-2 Toxin |
RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3801 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
EuroProxima Medroxyprogesterone Acetate |
A competitive enzyme immunoassay for screening and quantitative analysis of medroxyprogesterone acetate (MPA) in bovine kidney fat. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5131MPA |
EuroProxima Trenbolone |
This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRENBO |
EuroProxima Florfenicol-amine |
A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091FLOA |
EuroProxima Neomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of neomycin in milk, milk powder, tissue, honey, serum/plasma and urine samples. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5111NEO |
EuroProxima Oxytetracycline |
A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091OTC |
EuroProxima Tylosin |
EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151TYL |
EuroProxima Virginiamycin |
EuroProxima Virginiamycin is a competitive enzyme immunoassay for quantitative analysis of virginiamycin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151VIG |
EuroProxima Fluoroquinolones |
EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQG |
EuroProxima Fluoroquinolones II |
EuroProxima Fluoroquinolones II is a competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUQII |
EuroProxima Promazine, generic |
EuroProxima Promazine, generic is a competitive enzyme immunoassay for screening and quantitative analysis of several promazines in tissue, liver, urine and kidney. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201PROM |
EuroProxima Malachite Green Total/Crystal Violet |
EuroProxima Malachite Green Total/Crystal Violet is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5161MGT |
EuroProxima Azaperone-Azaperol |
EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201AZA |
EuroProxima Carazolol |
EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5201CARA |
EuroProxima Corticosteroid |
This competitive enzyme immunoassay is used for the quantitative determination of corticosteroids in milk, urine, muscle, liver and feed. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081COR |
EuroProxima Progesterone |
This competitive enzyme immunoassay is used for the quantitative determination of progesterone in milk and serum. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081PROG |
EuroProxima Stanozolol |
This competitive enzyme immunoassay is used for the quantitative determination of stanozolol in urine and faeces. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081STAN |
EuroProxima Triamcinolone |
This competitive enzyme immunoassay is used for the quantitative determination of triamcinolone in urine. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081TRIA |
EuroProxima Saxitoxin |
EuroProxima Saxitoxin is a competitive enzyme immunoassay for the screening and quantitative analysis of the shellfish poison saxitoxin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5191SAXI |
EuroProxima Lincomycin |
A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5151LIN |
EuroProxima Gentamicin |
A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5111GEN |
EuroProxima Flumequine |
EuroProxima Flumequine is a competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5101FLUM |
EuroProxima Erythromycin |
EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151ERY |
EuroProxima Domoic Acid |
EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5191DOMO |
EuroProxima Dimetridazole |
EuroProxima Dimetridazole is a competitive enzyme immunoassay for quantitative analysis of dimetridazole in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5091DIME |
EuroProxima Colistin |
A competitive enzyme immunoassay for screening and quantitative analysis of colistin in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5151COL |
EuroProxima Ivermectin |
EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | 5141IVER |
EuroProxima Diethylstilbestrol (DES) |
A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5081DES |
EuroProxima Penicillin |
A competitive enzyme immunoassay for screening and quantitative analysis of penicillins in various matrices. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | 5091PEN |
RIDASCREEN® Tetracyclin |
RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, … Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3505 |
RIDASCREEN® Chloramphenicol |
RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), … Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1511 |
RIDASCREEN® Sulfamethazin |
RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3011 |
RIDASCREEN® Streptomycin |
RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice. Continua a leggere |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3104 |