A competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp.
Malachite green (MG) and crystal violet (CV) are organic dyes used in the aquaculture industry as effective fungicides and disinfectants. Due to their toxic effects they are not authorised to be used in fish for human consumption. These dyes are in vivo metabolised to the reduced forms leucomalachite green (LMG) and leucocrystal violet (LCV). Within the EU, a Minimum Required Performance Limit (MRPL) of 2 ppb is set for the sum of MG and CV.
The MALACHITE GREEN TOTAL ELISA is a competitive enzyme immunoassay for the quantitative
analysis of MG, LMG, CV and LCV in fish and shrimp.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For fish and shrimp fast and efficient methods are included in the kit manual.|
|Incubation time||60 minutes|
|LOD (Detection limit)||Fish, shrimp: LOD 0.12ppb. CCbeta 0.3 ppb.|
Malachite green 100%, Leuco Malachite green 100%, Crystal violet 65%, Leucocrystal violet 65%.