Intended use:
The SureFood® ALLERGEN Beechnut is a real-time PCR for the direct qualitative detection of specific beechnut (Fagus sylvatica) DNA sequences in food.
General Information:
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time.
The technical verification of instruments was performed on Agilent Aria Dx, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
Accessories:
- SureFood® PREP Advanced
- SureFast® Mag PREP Food
- RIDA®CYCLER
- TANBead Maelstrom™ 8 Autostage
- TANBead Maelstrom™ 4800
| Article Numbers | S3628 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection limit) | The SureFood® ALLERGEN Beechnut PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1. |
| Instructions |
|---|
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